RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals

This hand-on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids.

In easy to follow step-by-step protocols you will learn

how RNAi works in plants, flies and mammals
how to design the most efficient RNAi constructs
how to achieve transient, stable and conditional RNAi in cell cultures
how to determine the efficiency of an RNAi experiment
how to use RNAi for gene therapy

All protocols have been thoroughly tested in the author's laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.

Includes a list of suppliers for RNAi reagents and equipment and a glossary of terms.

Ute Schepers RNA Interference Practice Principles Basics Methods Gene Silencing Celegans Drosophila Mammals

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